rabbit anti mouse il 17a  (Bioss)

Journal: PLOS ONE

Article Title: Effect of IL-17A on the immune response to pulmonary tuberculosis induced by high- and low-virulence strains of Mycobacterium bovis

doi: 10.1371/journal.pone.0307307

Figure Lengend Snippet: Primer sequences.

Article Snippet: Briefly, slides were incubated with rabbit IgG anti-mouse IL-17A (Santa Cruz Biotechnology, Santa Cruz, CA, USA; sc-7927) diluted 1:100 in the ImmunoDetector Protein Blocker/Antibody Diluent (Bio SB).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Exosomes from Ub‑HBcAg‑overexpressing dendritic cells induce T‑lymphocyte differentiation and enhance cytotoxic T‑lymphocyte activity

doi: 10.3892/etm.2023.11866

Figure Lengend Snippet: Isolation and identification of Dexs-Ub-HBcAg. (A) Transmission electron microscopy of the mDexs ultrastructure. Scale bar, 200 nm. (B) Expression of the positive exosomal markers CD9, CD63 and TSG101, and of HBcAg was examined via the western blot analysis of Dexs lysates. (C) Size dispersion profile of mDexs evaluated using ZetaVIEW ® nanoparticle tracking analysis, indicating a size peak of 123.8 nm. Dexs, dendritic cell-derived exosomes; HBcAg, hepatitis B virus core antigen; Dexs-Ub-HBcAg, Dexs loaded with ubiquitinated HBcAg; Con, control; TSG101, tumor susceptibility gene 101.

Article Snippet: Subsequently, the membrane was blocked with 5% non-fat milk at room temperature for 1 h. The primary antibodies used were rabbit anti-mouse CD63 (1:500; cat. no. ab216130; Abcam), anti-CD9 (1:500; cat. no. ab92726; Abcam) and tumor susceptibility gene 101 (TSG101; 1:500; cat. no. 102286-T38; SinoBiological) monoclonal antibodies.

Techniques: Isolation, Transmission Assay, Electron Microscopy, Expressing, Western Blot, Derivative Assay

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: Frequency of IFN-γ and IL-17A producing T cells in the salivary glands of male and female mice.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques:

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: Microengraving shows greater infiltration by IL-17A and IFN-γ producing cells in the salivary glands of SjS S mice. Quantification of T lymphocytes isolated from the major salivary gland of B6 male (●), B6 female (○), B6.NOD- Aec1Aec2 male ( ), and B6.NOD- Aec1Aec2 female ( ) mice expressing ( a ) CD4 + IFN-γ + , ( b ) CD8 + IFN-γ + , ( c ) CD4 + IL-17A + , ( d ) CD8 + IL-17A + , ( e ) CD4 + IFN-γ + IL-17A + , or ( f ) CD8 + IFN-γ + IL-17A + . The frequency in percentage of was determined by using the percentage (multiplied by 100) of the total number of CD4 + IFN-γ+ (Th1), CD4 + IL-17A+ (Th17), CD8 + IFN-γ+ (Tc1), CD8 + IL-17A+ (Tc17), CD4 + IL-17A+IFN-γ+ (Th17/1), and CD8 + IL-17A+IFN-γ+ (Tc1/17) cells from wells with single live cells among the total number of wells with single CD4 + or CD8 + cells. Group comparisons were made using a Kruskall-Wallis test with Dunn’s post hoc test. Statistical significance was defined as a p value < 0.05. All results are presented as means ± s.e.m. Significance was determined as *p < 0.05, **p < 0.01.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques: Isolation, Expressing

Journal: Scientific Reports

Article Title: Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren’s syndrome mice

doi: 10.1038/s41598-017-12627-6

Figure Lengend Snippet: MEME analysis of high frequency CDR3 regions from SjS S mice. Amino acid motif analysis was performed on the high frequency CDR3 sequences from the B6.NOD- Aec1/2 mice. Bit height corresponds to the likelihood of the amino acid in each position. Blue – hydrophobic, neutral amino acids, Red – positively charge hydrophilic amino acids, Green – Neutral hydrophilic amino acids, Magenta – negatively charged hydrophilic amino acids, Orange-glycine, Teal-Tyrosine, Pink-Histidine (positively charged moderately hydrophobic). E-value indicates the model confidence for the amino acid in that position. Cytokine indicates the secreted molecule(s) associated with the motif, V/J indicates recombination gene segment pairings, and % pop. Indicates the percent of the motif present in the IFN-γ or the IL-17A producing population. *Most popular V/J combination. **Motif located in both IL-17A and IFN-γ producing populations in both TCRα and TCRβ chains.

Article Snippet: The nanowells were hybridized with the capture slide containing antibodies against IL-17A (AAM60, AbD Serotec) and IFN-γ (505827, Biolegend).

Techniques: